Table 1.
Choline acetyl transferase (ChAT) activity (nanomoles of ACh formed per milligram of protein per hour, ±SEM) 6 weeks following i.c.v. administration of 192 IgG-saporin (TOX) or PBS. Depicted are group means of ChAT values in frontal, fronto-parietal, parieto-occipital, and occipital cortex, as well as cerebellum, dorsal hippocampus, entorhinal-piriform cortex and amygdala (NS normal saline, PHY physostigmine)
| PBS+NS
|
PBS+PHY
|
TOX+NS
|
TOX+PHY
|
All TOXIN
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Mean±SEM | n | Mean±SEM | n | Mean±SEM | n | Mean±SEM | n | %Decrease | |
| Frontal | 46.78±3.67 | 5 | 47.04±0.85 | 6 | 8.93±1.05** | 5 | 9.44±1.17**** | 6 | 80 |
| Fronto-parietal | 39.16±2.96 | 5 | 44.46±1.97 | 6 | 9.28±4.59** | 5 | 6.50±0.65**** | 6 | 82 |
| Parieto-occip. | 36.97±1.80 | 5 | 39.43±1.44 | 6 | 5.91±0.70** | 5 | 6.61±0.62**** | 6 | 84 |
| Occipital | 51.43±8.26 | 5 | 46.49±1.81 | 6 | 6.64±0.74** | 4 | 9.34±1.34**** | 6 | 83 |
| Cerebellum | 3.91±0.68 | 5 | 4.40±0.57 | 6 | 3.89±0.63 | 5 | 4.28±0.43 | 6 | 2 |
| Ent-pyrif-amyg. | 83.79±7.69 | 4 | 77.22±12.99 | 5 | 49.34±8.17* | 4 | 48.01±8.04*** | 5 | 39 |
| Dorsal hippoc. | 44.25±6.05 | 5 | 44.79±2.43 | 6 | 2.86±0.63** | 4 | 3.56±0.79**** | 6 | 93 |
| Ent-pyrif.a | 76.7±6.17 | 9 | 44.40±4.08* | 6 | 42 | ||||
| Amygdalaa | 90.4±3.41 | 7 | 65.00±7.42* | 5 | 28 | ||||
*
P<0.01,
**
P<0.001 for TOX+NS vs PBS+NS;
***
P<0.01 or
****
P<0.001 for TOX+PHY vs PBS+PHY
a
EEG recordings were not made from animals in these groups