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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 25;0:207–216. doi: 10.1016/j.jchromb.2013.11.041

Table 1.

SRM conditions for the prostanoid metabolites and the deuterated internal standards in negative electrospray ionization mode.

Analyte Precursor → Product (m/z) Collision Energy Retention time (min)
6-keto-PGF1α 369.3 → 245.1 28 1.61
6-keto-PGF1α-d4 373.2 →249.2 25 1.60
11b-PGF2α 353.1 → 193.1 26 2.30
11b-PGF2α-d4 357 → 197 27 2.29
PGF1α 355.1 → 311.1 23 2.63
PGF1α-d9 364 → 320.1 25 2.62
PGF2α 352.9 → 309.1 23 2.68
PGF2α-d4 357 → 313.1 23 2.66
PGE2 351.1 → 271.1 20 2.90
PGE2-d4 355.1 → 275.1 19 2.88
11-dehydro-TXB2 367 → 305.1 22 3.00
11-dehydro-TXB2-d4 371 → 309.1 20 2.98
PGD2 351.1 → 271.1 20 3.30
PGD2-d4 355.1 → 275.1 17 3.29
PGA2 333 → 271.2 19 6.00
PGJ2 333 → 271.2 19 6.42
15-deoxy-Δ12,14-PGD2 333 → 271.2 19 9.32
15-deoxy-Δ12,14-PGJ2 316 → 272 17 9.64
15-deoxy-Δ12,14-PGJ2-d4 320 → 276 17 9.63