Table 2.
Accuracy and precision of assay, represented by quality controls, for 11 prostanoid metabolites extracted in deionized water. Intra-assay relative standard deviation (RSD) ranged from 1.45 to 11.9% and inter-day RSD ranged from 1.76 to 14.1%.
| Analyte | Spiked amount (pg on column) | Intra-assay concentration (mean±s.d) (pg on column) | RE% | RSD% | Inter-assay concentration (mean±s.d) (pg on column) | RE% | RSD% |
|---|---|---|---|---|---|---|---|
| 6-keto-PGF1α | 40 | 38.7 ± 2.26 | -3.01 | 5.82 | 40.7 ± 3.53 | 1.86 | 8.67 |
| 450 | 437 ± 16.7 | -2.83 | 3.82 | 449 ± 25.5 | -0.21 | 5.69 | |
| 1250 | 1209 ± 34.2 | -3.25 | 2.83 | 1241 ± 63.1 | -0.72 | 5.08 | |
| 11β-PGF2α | 40 | 41.2 ± 2.91 | 3.00 | 7.06 | 40.9 ± 4.01 | 2.43 | 9.78 |
| 450 | 435 ± 21.4 | -3.12 | 4.91 | 437 ± 15.7 | -2.80 | 3.58 | |
| 1250 | 1201 ± 52 | -3.88 | 4.33 | 1228 ± 57 | -1.71 | 4.64 | |
| PGF1α | 40 | 37.7 ± 3.23 | -5.72 | 8.56 | 39.5 ± 3.50 | -1.28 | 8.86 |
| 450 | 430 ± 15.3 | -4.39 | 3.49 | 438 ± 11.1 | -2.56 | 2.54 | |
| 1250 | 1216 ± 58.4 | -2.68 | 4.80 | 1228 ± 64.5 | -1.69 | 5.25 | |
| PGF2α | 40 | 38.9 ± 4.65 | -2.55 | 11.9 | 38.3 ±5.41 | -4.25 | 14.1 |
| 450 | 467 ± 47.7 | 3.90 | 10.2 | 455 ± 25.7 | 1.15 | 5.65 | |
| 1250 | 1249 ± 131 | -0.06 | 10.5 | 1221 ± 67 | -2.27 | 5.49 | |
| PGE2 | 40 | 39.6 ± 1.58 | -1.00 | 3.99 | 38.9 ± 1.24 | -2.71 | 3.19 |
| 450 | 413 ± 17.7 | -8.11 | 4.29 | 439 ± 16.8 | -2.23 | 3.83 | |
| 1250 | 1175 ± 44.8 | -5.99 | 3.81 | 1209 ± 52 | -3.26 | 4.30 | |
| 11-dehydro-TXB2 | 40 | 35.0 ± 1.88 | -12.4 | 5.38 | 38.8 ± 4.05 | -2.84 | 10.4 |
| 450 | 407 ± 26.5 | -9.41 | 6.50 | 446 ± 31.4 | -0.88 | 7.04 | |
| 1250 | 1314 ± 61.2 | 5.14 | 4.66 | 1363 ± 77.8 | 9.05 | 5.71 | |
| PGD2 | 40 | 39.7 ± 3.12 | -0.74 | 7.85 | 39.9 ± 1.89 | -0.02 | 4.71 |
| 450 | 412 ± 5.97 | -8.24 | 1.45 | 436 ± 17.8 | -3.03 | 4.09 | |
| 1250 | 1206 ± 49.2 | -3.46 | 4.08 | 1238 ± 21.7 | -0.91 | 1.76 | |
| PGA2 | 40 | 37.6 ± 2.94 | -5.83 | 7.80 | 39.0 ± 2.67 | -2.36 | 6.83 |
| 450 | 409 ± 22.6 | -9.03 | 5.53 | 434 ± 30.7 | -3.54 | 7.08 | |
| 1250 | 1192 ± 112 | -4.57 | 9.41 | 1228 ± 69.6 | -1.71 | 5.67 | |
| PGJ2 | 40 | 41.1 ± 2.72 | 2.87 | 6.60 | 39.5 ± 2.09 | -1.18 | 5.28 |
| 450 | 455 ± 11.2 | 1.25 | 2.46 | 443 ± 24.7 | -1.38 | 5.57 | |
| 1250 | 1267 ± 47.6 | 1.37 | 3.76 | 1237 ± 56.7 | -1.01 | 4.59 | |
| 15-deoxy-PGD2 | 40 | 38.1 ± 4.11 | -4.58 | 10.7 | 39.8 ± 2.50 | -0.40 | 6.26 |
| 450 | 430 ± 35.5 | -4.22 | 8.24 | 442 ± 28.6 | -1.72 | 6.47 | |
| 1250 | 1206 ± 113 | -3.44 | 9.44 | 1249 ± 76.1 | -0.02 | 6.09 | |
| 15-deoxy-PGJ2 | 40 | 42.0 ± 3.54 | 5.18 | 8.41 | 41.6 ± 2.96 | 4.11 | 7.10 |
| 450 | 443 ± 17.9 | -1.54 | 4.05 | 449 ± 13.9 | -0.08 | 3.10 | |
| 1250 | 1216 ± 95.6 | -2.72 | 7.86 | 1253 ± 40.6 | 0.27 | 3.25 |