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Extraction recovery (E.R) efficiency of deuterated prostanoid metabolites were measured at 12.5 ng/ml (750 pg on column) in deionized water. Recovery efficiencies ranged from 88 to 100% for all metabolites with relative standard deviations below 18%.
| Analyte | Extraction Efficiency (%) | RSD (%) |
|---|---|---|
| 6-keto-PGF1α-d4 | 96.9 | 15.6 |
| 11β-PGF2α-d4 | 88.1 | 15.5 |
| PGF1α-d9 | 100.3 | 13.5 |
| PGF2α-d4 | 93.7 | 14.9 |
| PGE2-d4 | 91.6 | 16.3 |
| 11-dehydro-TXB2-d4 | 89.8 | 18.0 |
| PGD2-d4 | 89.5 | 14.6 |
| 15-deoxy-Δ12,14-PGJ2-d4 | 91.4 | 12.2 |
