. Author manuscript; available in PMC: 2015 Jan 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 25;0:207–216. doi: 10.1016/j.jchromb.2013.11.041

Table 4.

Assessment of matrix effects of the deionized water on the reproducibility of the assay. Data are represented as coefficient of variation (%CV) of the peak area of each deuterated metabolite of the post-extraction spike in deionized water along with the CV of neat samples prepared in 80:20 methanol:water (n=6). Similarly matrix effect (M.E) of deionized water on prostanoids was also measured. Data are represented as the average M.E values and the CV for deionized water spiked with deuterated metabolites at 12.5 ng/ml (n=6). Results indicate minimal effects of the deionized water on the reproducibility and reliability of the assay to measure prostanoids.

Analyte	Precision (%CV)		Matrix Effect (M.E)

	Neat	Deionized Water	Mean	%CV
6-keto-PGF_1α-d4	1.28	15.8	0.89	14.7
11β-PGF_2α-d4	8.50	11.0	0.94	13.7
PGF_1α-d9	12.8	14.2	0.97	18.4
PGF_2α-d4	12.4	9.1	0.91	15.0
PGE₂-d4	7.03	11.7	0.94	12.7
11-dehydro-TXB₂-d4	5.38	13.8	0.86	13.9
PGD₂-d4	8.02	10.9	0.87	13.0
15-deoxy-Δ12,14-PGJ₂-d4	6.16	7.61	0.93	9.96