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. Author manuscript; available in PMC: 2015 Aug 8.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Jul 10;450(4):1427–1432. doi: 10.1016/j.bbrc.2014.07.013

Fig 4.

Fig 4

Generation of chromosomal PfSPP-GFP chimera and its effect on parasite life cycle. (A) Schematic illustration of PfSPP vector with GFP inserted at the C-terminus. The integration event produced a full-length PfSPP coding frame fused to GFP. The bottom panel shows parasites displaying GFP fluorescence along with the DIC images of live parasites. (B) A montage of gametocyte-like parasites recovered after transfection and selection in the presence of WR99210. (C) Quantification of HSP101 cleavage activity by PfSPP and PfSPP-GFP constructs.