Pam2CSK4 primed BMDC from C57BL/6, littermate Rip3−/−, Casp8−/−Rip3−/− mice were stimulated with β-glucans for 24h, FasL, pdAdT for 6h (A) or as indicated (B). IL-1β released in the supernatants was measured by ELISA (A, B). Cells were stimulated as indicated and precipitated supernatants and lysates were probed for IL-1β, caspase-1 or caspase-8 (C, D). Statistical analysis was performed on results in panels A and B as described in the Materials and Methods section.