Figure 2. Effect of BK channel modulators on leptin-induced neuroprotection in rat cortical neurons.
(A, B) Concentration-dependent effect of the BK blockers paxilline (A, Pax) and iberiotoxin (B, Ibtx) on leptin-induced neuroprotection of 100 μM NMDA-induced neuronal death. (C) Concentration-dependent effect of the BK channel activator NS1619 on NMDA-induced neuronal death. In each panel, the bars on the left indicate the drug exposure protocol. Neuronal viability was measured 24 hrs after the insult with the MTT assay. Data are expressed as percentage of neuronal viability in untreated, control neurons. Each data point is the mean ± S.E.M. of five separate experiments, each performed in triplicate. Asterisks denote values statistically different from respective controls (NMDA alone; P < 0.05).