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. Author manuscript; available in PMC: 2014 Aug 17.
Published in final edited form as: Mol Cell Neurosci. 2014 Apr 28;0:1–12. doi: 10.1016/j.mcn.2014.04.006

Figure 4.

Figure 4

Control experiments demonstrate the specificity of the FISH signals.

(A) miR-124 signal is drastically reduced in the presence of a fully complementary competitive inhibitor at 10X concentration. Red, miR-124; green, GFAP; cyan, MAP2; blue, Hoechst. Scale bar = 20 μm. (B) miR-124 signal is absent from astrocytes. Red, miR-124; green, GFAP; cyan, MAP2; blue, Hoechst. (C) GluA2 mRNA signal is absent from astrocytes. Red, GluA2 mRNA; green, GFAP; cyan, MAP2; blue, Hoechst. (D) Camk2α mRNA signal is absent from inhibitory neurons. Red, Camk2α mRNA; green, GAD67; white, MAP2.