Table 1.
mRNA for factor D, MASPs and cytokines in the knee joints of WT mice with and without CAIA1
| mRNA | No CAIA2 | PBS | AdGFP | AdhMAp44 LD3 | AdhMAp44 HD4 |
|---|---|---|---|---|---|
| Factor D | 28.27 ± 10.7 | 1523.6 ± 523.8 | 709.8 ± 73.9 | 295.31 ± 52.2 | 324.0 ± 120.5 |
| p | 0.0017 | 0.018 | |||
| Decrease | 58% | 54% | |||
| MASP-1 | 6.83 ± 0.9 | 40.6 ± 523.8 | 51.3 ± 3.7 | 31.8 ± 1.6 | 22.7 ± 3.2 |
| p | 0.001 | 0.001 | |||
| Decrease | 38% | 56% | |||
| MASP-3 | 1.38 ± 0.13 | 16.6 ± 2.7 | 14.2 ± 0.13 | 8.9 ± 0.76 | 5.2 ± 0.55 |
| p | 0.025 | 0.0035 | |||
| Decrease | 38% | 56% | |||
| MASP-2 | 0.39 ± 0.09 | 1.3 ± 0.15 | 1.2 ± 0.13 | 0.73 ± 0.1 | 0.53 ± 0.2 |
| p | 0.044 | 0.010 | |||
| Decrease | 39% | 56% | |||
| MBL-A5 | 0.29 ± 0.09 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.01 ± 0.004 | 0.01 ± 0.004 |
| MBL-C5 | 1.13 ± 0.27 | 0.002 ± 0.002 | 0.016 ± 0.006 | 0.006 ± 0.002 | 0.003 ± 0.002 |
| FCN-A | 145.1 ± 34.2 | 366.3 ± 55.3 | 295.6 ± 34.1 | 170.0 ± 32.6 | 117.3 ± 30.1 |
| p | 0.028 | 0.069 | |||
| Decrease | 42% | 60% | |||
| TNF-α | 1.06 ± 0.134 | 15.1 ± 2.1 | 12.8 ± 2.1 | 3.09 ± 0.6 | 3.9 ± 0.37 |
| p | 0.002 | 0.007 | |||
| Decrease | 76% | 66% | |||
| IL-1α | 1.36 ± 0.19 | 4.7 ± 1.2 | 4.0 ± 0.75 | 0.99 ± 0.23 | 0.53 ± 0.14 |
| p | 0.005 | 0.005 | |||
| Decrease | 75% | 87% | |||
| IL-1β | 2.91 ± 0.50 | 41.3 ± 6.1 | 29.8 ± 8.5 | 5.2 ± 0.50 | 4.40 ± 0.2 |
| p | 0.02 | 0.03 | |||
| Decrease | 83% | 85% |
Data are expressed in pg/ng 18s rRNA with mean ± SEM based on the indicated number of mice (n). CAIA mice treated with PBS (n=5), AdGFP (n = 5), AdhMAp44 LD (n = 5) and AdhMAp44 HD (n =5).
Baseline levels for various mRNA targets from the knee joint of WT mice without CAIA and without any treatment (n = 4).
LD = Low dose
HD = high dose.
The mRNA levels of MBL-A and MBL-C were very low.
Liver from WT mice used as a positive control to measuring the mRNA levels (data not shown). All p-values for different mRNAs in mice treated either with AdhMAp44 LD or with AdhMAp44 HD were compared with the corresponding values of WT mice treated with AdGFP. The percent (%) decrease in mRNA levels in mice treated with AdhMAp44 in each column has been shown compared with mice treated with AdGFP. p values were compared between mice treated either with AdGFP and AdhMAp44 LD or AdhMAp44 HD. p < 0.05 were considered statistically significant.