Fig. 1.

A bioinformatics approach for the identification of targeted mutation regions within spike glycoprotein of MHV/A59. (A) Schematic representation of the MHV spike protein structure. The following features are present: RBD – receptor binding domain, PFP – putative fusion peptide, PEP – putative fusion domain, HR – hydrophobic repeats, TD – transmembrane domain, and CT – cytoplasmic tail. The location of the proline residue at position 939 within the putative fusion peptide is also shown in the schematic. An expansion of the region of weak sequence similarity between MHV S protein and the FcγRII protein is shown below the schematic. Residues of sequence identity are shown in bold underlined lower case text. (B) Amino acid sequence comparison of MHV/A59, BCV and HCoV-OC43 spike proteins. Amino acids that were identical in the MHV-A59 and BCV spike proteins but differed from the HCoV-OC43 S protein are in a bold and underlined font.