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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2014 Jul 28;193(5):2238–2247. doi: 10.4049/jimmunol.1400604

Figure 2.

Figure 2

Effect of GITR ligation on in vitro cytokine production and phenotype of CD4+CD25+ and CD4+CD25− T cells. Enriched CD4+CD25+ and CD4+CD25− T cells were cultured in medium alone, in the presence of GITRL, or phorbol/ionomycin. Supernates were recovered for cytokine production and changes in surface marker phenotype were analyzed by FACS. A: Levels of cytokines following 72 hr culture. †p<0.05 comparing basal levels in medium (Med). *p<0.05 comparing levels following GITRL stimulation. #p<0.05 comparing levels in the presence of phorbol/ionomycin (PI). B: FACS analysis following GITRL stimulation.