Figure 1. Zfp318 is highly expressed in mature follicular B cells and is conditionally deleted via Vav-Cre.

(A) RT-PCR of cDNA derived from the indicated B cell subsets. Four biological replicates were assayed for each subset. NF = Newly Formed, T1 = Transitional 1, T2 = Transitional 2, FO = Follicular, MZ = Marginal Zone; Statistics: * p < 0.05, ** p < 0.01, *** p < 0.001 (B) Schematic diagram of the Zfp318^Fl/Fl conditional allele. Cre-mediated recombination of LoxP sites results in the deletion of the Zfp318 proximal promoter as well as exons 1 and 2 (black boxes). (C) Representative genotyping of animals possessing targeted alleles (fl/fl) or heterozygous alleles (WT/fl) in the presence or absence of the Vav-Cre deleter construct. Analysis of genomic DNA isolated from the tail or bone marrow shown using primer set P1/P2 (Supplemental Table 1). The Zfp318^fl/fl (Zfp318 KN) allele is only deleted in cells isolated from the bone marrow demonstrating the hematopoietic specificity of gene deletion. (D) Loss of Zfp318 transcripts was validated using cDNA derived from WT and cKO total spleen tissues. Three biological replicates were performed per genotype. ND = Not Detected; For (A) and (D), Zfp318 transcripts were normalized to Actb and data is represented as the mean ± SEM.