Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2014 Jul 23;193(5):2546–2553. doi: 10.4049/jimmunol.1401275

Figure 3. Deletion of Zfp318 abrogates surface expression of IgD on B cells.

Figure 3

(A-C) Representative mean fluorescence intensity histograms for WT, heterozygous (HET) and knockout (cKO) animals. (A) CD35 and (B) CD21 and CD35 are equally expressed on the surface of splenic CD19+ CD23+/− B cells from all genotypes. (C) B220 is properly expressed by splenic CD19+ B cells and is still repressed by CD19-non-B cell spleen populations. (D-E) Representative mean fluorescence intensity histograms for WT, heterozygous (HET) and knockout (cKO) splenic B cell populations. Upon deletion of Zfp318, (D) IgM surface expression is upregulated and (E) IgD surface expression is abrogated. (F) Quantification of geometric mean fluorescence intensities of B cell populations analyzed in (D) and (E). (G) RT-PCR analysis of total spleen cDNA from WT and cKO animals. Three animals per genotype were analyzed. Ighm (top panel) and Ighd (bottom panel) transcripts were normalized to Pax5 to compensate for any variance in total B cell contribution. Expression of Ighd was reduced >90% in the cKO spleen. Statistics for (F) and (G): * p < 0.05, ** p < 0.01, **** p < 0.0001