Figure 4. RNA-Seq analysis of CD19+ splenic B cells from WT and Zfp318 deficient animals.

(A) Transcriptome analysis of WT and cKO splenic CD19+ B cells. Three replicates per genotype were sequenced and analyzed (Supplemental Table 3). On the left, Log₂ Fold Change per gene between WT and cKO samples is plotted against the P-value. On the right, WT and cKO FPKM are plotted for all genes analyzed. Genes were considered significantly altered with a Log₂ Fold Change >2 and a P-value < 0.01. (B-D) Representative custom tracks (Left) and RT-PCR validation (Right) for selected genes of interest. (B) Zfp318 expression is lost in the knockout samples. (C) The alternatively spliced Cr2 locus demonstrates equivalent expression in WT and cKO CD19+ B cells. (D) Expression of the Ighm/Ighd locus is significantly impaired in the cKO specific to the regions for the IgD-encoding exons (Ighd) (>95% reduction). Statistics: * p < 0.05