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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2014 Jul 23;193(5):2546–2553. doi: 10.4049/jimmunol.1401275

Figure 5. Deletion of Zfp318 blocks transcription downstream of the terminal Ighm-specific exon.

Figure 5

(A) Representative RNA-Seq custom tracks from WT and cKO CD19+ B cells illustrating Ighm-specific exons 5-6, the intervening intronic region and Ighd-specific exons 1-2. Primer sets used to quantify transcription throughout the Ighm locus are indicated. (B) LightCycler-based RT-PCR quantification of transcription downstream of Ighm-specific exon 6. (Left) For each sample, Ct-derived transcript ratios of SetB/SetA were generated. The mean WT value was used to derive the “percentage of WT” for each individual sample (e.g. WT-1/WT-mean or cKO-1/WT-mean). Statistics: ** p < 0.01 (Right) Amplification products from a Cd3ε upstream non-coding intergenic region (Top) and from a region internal to IgM-specific exon 6 (Set C) (Bottom).