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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2014 Jul 23;193(5):2546–2553. doi: 10.4049/jimmunol.1401275

Figure 6. Model of Zfp318 transcriptional regulation throughout the Ighm locus.

Figure 6

(A-C) Quantification of (A) Zfp318, (B) Ighm and (C) Ighd coding transcripts within the indicated B cell subsets. Panel A was reproduced from Fig. 1A. NF = Newly Formed, T1 = Transitional 1, T2 = Transitional 2, FO = Follicular, MZ = Marginal Zone; Statistics: * p < 0.05, ** p <0.01, *** p < 0.001 In (B), # represents comparisons between the indicated cell type and the T2 population with # p <0.05 and ### p < 0.001. In (C), $ represents comparisons between the indicated cell type and the FO population with $$$ p < 0.001. (D) Stylized depiction of transcription throughout the Ighm/Ighd locus (not drawn to scale). In the presence of Zfp318 (Top), transcriptional termination is blocked and transcription continues past the terminal Ighm-specific exon. This allows for splicing to Ighd-specific exons and generation of a mature IgD protein. Upon the loss of Zfp318 (Bottom), transcription is terminated at the final Ighm-specific exon. As a result, the downstream Ighd-specific exons cannot be transcribed or spliced into a mature transcript.