Fig. 5.

A′B′ and E′F′ loop MNV-1 P domain mutants do not mediate escape from mAbs 2D3.7 and 4F9.4 neutralization. WT MNV-1 and A′B′ loop mutants with IgA mAb 2D3.7 (a), A′B′ loop mutants with IgA mAb 4F9.4 (b), E′F′ loop mutants with IgA mAb 2D3.7 (c) and E′F′ loop mutants with IgA mAb 4F9.4 (d) were subjected to in vitro neutralization. Virus was incubated with the indicated concentrations of mAbs 2D3.7 and 4F9.4 for 30 min at 37 °C before performing plaque neutralization assays. The percentage infectivity was calculated by setting WT MNV-1 without mAb to 100 %. The dashed lines indicate 20 and 80 % infectivity. Data are presented as means±sem of three independent experiments.