Table 2. Restriction enzymes utilized for generating full-length DNA templates for in vitro transcription.
| Chimera | T7/WNV 5′UTR | 5′ Cut | 3′ Cut | WNV 3′UTR | 5′ Cut | 3′ Cut |
| WNV-NY/MAD78 | pA-NY | MluI | NgoMIV | pB-MAD | NgoMIV | XbaI |
| WNV-MAD78/NY | pA-MAD | MluI | NgoMIV | pB-NY | NgoMIV | XbaI |
| WNV-NYMAD78(5′UTR→prM) | pA-NYMAD78(5′UTR→prM) | MluI | NgoMIV | pB-NY | NgoMIV | XbaI |
| WNV-NYMAD78(E) | pA-NYMAD78(E) | MluI | NgoMIV | pB-NY | NgoMIV | XbaI |
| WNV-NYS156P | PCR-NYS156P | blunt | blunt | pFL-NY | SphI | XbaI |
| WNV-MAD78P156S | pI-MADP156S | MluI | BamHI | WNV-MADIC | BamHI | NotI |