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. 2014 Sep;95(Pt 9):2038–2049. doi: 10.1099/vir.0.065664-0

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Interaction between N1 and Ub in transfected HEK 293T cells. (a) Immunoprecipitation of FLAG-tagged Ub in the presence (+) or absence (–) of HA-tagged N1. Whole-cell lysates (WCL) and FLAG immunoprecipitates (FLAG IP) were resolved in Novex 4–12 % Bis-Tris protein gels and immunoblotted (IB) with the indicated antibodies. The positions of molecular mass markers are indicated. (b) Interaction between FLAG-tagged versions of GFP, N1 or Ub and Rluc fused to N1 (Rluc-N1) or RLuc was assessed by LUMIER. Ratios between luciferase activity in lysates and eluates were calculated for each condition and plotted as a binding fold over control reactions. Data shown are from one experiment of three experiments showing indistinguishable results.