Table 1.

Post-translational modifications of biopharmaceuticals expressed in the secretory system of microalgae.

Expressed protein	Promoter	N-glycosylation sitea	Glycosylationb	References
IN C. REINHARDTII
Human EPO	Hsp70A/RbcS2	Yes	Yes	Eichler-Stahlberg et al., 2009
IN P. TRICORNUTUM
Monoclonal human IgG against the Hepatitis B surface antigen	Nitrate reductase	Yes	Yes	Hempel et al., 2011b; Hempel and Maier, 2012
Hepatitis B surface antigen	Nitrate reductase	Yes	No	Hempel et al., 2011b
IN D. SALINA
Hepatitis B surface antigen	Maize Ubiquitin + Ω TMV enhancer	Yes	No	Geng et al., 2003

^aPredicted N-glycosylation sites by bio-informatic analysis of the protein sequence.

^bExperimental evidence of the presence of N-glycans attached to the N-glycosylation site.