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. Author manuscript; available in PMC: 2015 Feb 5.
Published in final edited form as: Nat Commun. 2014 Aug 5;5:4603. doi: 10.1038/ncomms5603

Figure 3. DOCK8 is also required for the IL-23-mediated induction of IL-22.

Figure 3

(a) SI LPLs from WT and DOCK8pri/pri mice were stimulated with 20 ng/ml IL-23 for 4 hours in the presence of Golgi stop. RORγt and IL-22 expression in CD3− cells was analyzed by flow cytometry. (b) Percentage and absolute numbers of intestinal CD3−IL-22+ cells. (c) Sorted ILC3 (CD90.2+IL-23Rgfp/+) from intestinal LPLs of Rag1−/−IL-23Rgfp/+ and Rag1−/−IL-23Rgfp/+DOCK8pri/pri mice were cultured with or without IL-23 (20ng/ml). The IL-22 produced in culture supernatant was measured by ELISA. (d) FACS analysis for detection of pSTAT3 in ILC3 from Rag1−/−IL-23Rgfp/+ or Rag1−/−IL-23Rgfp/+DOCK8pri/pri mice. All the data are representative of three independent experiments with three mice per group. The data shown are the mean ± SD. P-values were obtained by student’s t-test. **p< 0.01, ***p< 0.001.