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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Biochem J. 2014 Mar 15;458(3):459–467. doi: 10.1042/BJ20131161

Figure 1. Ubiquitinated proteins accumulate in strains lacking VMS1 or expressing a form of Vms1 that is defective for Cdc48 interaction.

Figure 1

(A) The indicated yeast strains were grown to log-phase in rich medium, and total protein was precipitated and resolved by SDS/PAGE. Western blot analysis was used to detect protein-conjugated ubiquitin species (Ub) and GPD (glycerol-3-phosphate dehydrogenase), which served as a loading control. (B) Wild-type (WT) or vms1Δ yeast strains harbouring an empty vector (−), or vectors engineered for the expression of full-length VMS1 (Vms1 HA) or a mutant lacking the VIM domain (ΔVIM HA) were grown in minimal medium to log-phase and processed as in (A) for Western blot analysis. (C) The levels of ubiquitinated proteins > 72 kDa in part (B) were quantified. P values are shown for specific pairwise comparisons.