Figure 16.
Dataset 6, 600 frames,“most” movement at depth z0 = 25.000 μm. (a) A frame of the original t-stack. (b) The template used for performing motion correction. (c) The template (red channel) superimposed on the MIP (green channel) of the uncorrected t-stack. (d) Hysteresis results of the template superimposed with a frame of the uncorrected t-stack. (e) The template (red channel) superimposed on the MIP of the motion corrected t-stack (green channel) using the baseline algorithm. (f) The template (red channel) superimposed on the MIP of the motion corrected t-stack (green channel) using the presented algorithm; notice the near perfect overlap in yellow and the sharpness of all the vessels. Dataset 6 primarily exhibits rigid motion and both the baseline and the presented algorithm perform well as seen in images e and f.