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Basophil activation assay. Rat basophilic leukemia cells were sensitized with one serum from the Bet v 1-sensitized group (serum 4) and with three sera from the panel of mung bean sprout sensitized patients (S2, S4, and S9), and then incubated with Bet v 1, Vig r 1, and Vig r 6. Activation was determined by measuring β-hexosaminidase activity in the supernatants.
