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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Sci Signal. 2014 May 20;7(326):ra47. doi: 10.1126/scisignal.2005070

Fig. 2. AR transcriptionally suppresses MYB through modification of chromatin architecture on MYB promoter.

Fig. 2

(A) The structural illustration of different MYB promoter luciferase reporters. (B) Luciferase reporter assays. LNCaP cells were transfected with MYB promoter reporters or control pGL3-basic empty vector and β-gal–expressing vector (internal control) for 24 h, followed by treatment with CSS or CSS plus 10 nM R1881 (CSS+R) for 24 h. (C) ChIP assays. LNCaP cells were treated with or without 10 nM R1881 in CSS medium for 24 h prior to ChIP assays. The data are expressed as percentage of input. Pol II, RNA polymerase II; LSD1, lysine-specific demethylase 1; M3H3K27, trimethyl-histone 3 lysine 27; M3H3K4, trimethyl-histone 3 lysine 4; AceH3, acetyl-histone 3 lysine 27. *P < 0.05.