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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Sci Signal. 2014 May 20;7(326):ra47. doi: 10.1126/scisignal.2005070

Fig. 5. DDR gene silencing synergizes with PARP inhibition to increase cytotoxicity to PCa cells.

Fig. 5

(A) Western blotting analysis showing efficiency of MYB, BRCA1 and TOPBP1 siRNAs. Note MYB gene silencing led to downregulation of BRCA1 and TopBP1. (B–E), Cell cycle analysis. PCa cells were untreated, mock transfected or transfected with specific siRNAs (20 nM) and grown for 24 h, and then followed by the treatment of PARP inhibitor olaparib (OLA; 10 μM) for 48 h. Top panels are representative cell cycle profiles. Bottom bar graphs are average of triplicates. C, vehicle control; O, olaparib; Data are presented as mean ± standard deviation. # indicates synergistic effect. Synergy quantification was summarized in Supplementary Table S4.