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. Author manuscript; available in PMC: 2014 Aug 18.
Published in final edited form as: J Neuropathol Exp Neurol. 2011 Jan;70(1):69–82. doi: 10.1097/NEN.0b013e318203ae74

Figure 3.

Figure 3

Vanishing white matter disease (VWM) astrocytes express oligodendrocytic lineage-specific proteins. (A, B) Double stain for glial fibrillary acidic protein (GFAP) (A, patient VWM3) or vimentin (B, patient VWM99) and galactocerebroside (GalC) show dysmorphic astrocytes with homogeneous cytoplasmic GalC immunoreactivity extending far into the processes. (C) In astrocytes from VWM patients, GalC colocalizes with other astrocytic lineage specific-markers, including GFAPδ (patient VWM44). (D) GFAP-positive cells with astrocyte morphology also express olig2 with a cytoplasmic immunoreactivity pattern (patient VWM80). E: Primary culture of sorted glial progenitors shows a cell with both nuclear and cytoplasmically translocated olig2 and morphology and CD44-immunoreactivity, consistent with an astrocyte progenitor; 2 oligodendrocyte lineage-committed cells only express nuclear olig2 (patient VWM576). In all panels, the nuclei are stained with DAPI (blue). Original magnifications: A, E, 200×; B–D, 400×.