Table 2.
Antibodies and q-PCR Primers
| Antibody | Marker | Dilution | Vendor | Catalog no. |
|---|---|---|---|---|
| Ki67 (Mib-1) | Cell proliferation | 1:400 | Neomarkers | clone SP6, RM-9106-S1 |
| GFAP | Astrocytes | 1:1000 | Millipore | AB5541 |
| S100β | Astrocytes | 1:200 | Sigma | S2532 |
| Vimentin | Astrocytes | 1:2000 | clone V9 (a) | |
| Nestin | Astrocytes | 1:200 | Millipore | MAB5623 |
| GFAPδ | Astrocytes | 1:500 | (b) | |
| αB-crystallin | Astrocytes | 1:1000 | Novocastra | ABCRYS-512-U |
| CD44 (Hermes3) | Astrocyte progenitors | 1:1000 | (c) | |
| Olig2 | OPC/Oligodendrocytes | 1:400 | Abcam | ab33427 |
| PDGFRα (CD140a) | OPC | 1:100 | BD Pharmigen | 556001 |
| NG2 | OPC | 1:100 | Millipore | MAB2029 (clone 9.2.27) |
| GalC (O1) | Oligodendrocytes | 1:30 | Millipore | AB142 |
| GalC (O1) | Oligodendrocytes | 1:50 | Sigma-Aldrich | O7014 |
| GAPDH | Constitutively expressed | 1:500 | Abcam | Ab9485 |
| q-PCR primer (5’-3’) | Sequence | |||
| GFAPδ, forward | CCTACAGGAAGCTGCTAGAG | |||
| GFAPδ, reverse | GCGTTCCATTTACAATCTGGT | |||
| Total GFAP, forward | AGAAGCTCCAGGATGAAACC | |||
| Total GFAP, reverse | TTCATCTGCTTCCTGTCTATAGG | |||
| GFAPα, forward | AGAGGTCATTAAGGAGTCCA | |||
| GFAPα, reverse | CAACTATCCTGCTTCTGCTC | |||
| GAPDH, forward | CTCTCTGCTCCTCCTGTTCGAC | |||
| GAPDH, reverse | TGAGCGATGTGGCTCGGCT | |||
| HPRT, forward | ATGGGAGGCCATCACATTGT | |||
| HPRT, reverse | ATGTAATCCAGCAGGTCAGCAA | |||
| RPLP0, forward | GTGATGTGCAGCTGATCAAGACT | |||
| RPLP0, reverse | GATGACCAGCCCAAAGGAGA | |||
| CypB, forward | AAGGACTTCATGATCCAGGG | |||
| CypB, reverse | TGAAGTTCTCATCGGGGAAG | |||
(a)
Antibody manufactured at the Department of Pathology, VU University Medical Center, Amsterdam, the Netherlands.
(b)
Antibody manufactured at the Netherlands Institute for Neurosciences, Amsterdam, the Netherlands (ref. 17).
(c)
Antibody kind gift of E. Aronica, University of Amsterdam, the Netherlands.