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. Author manuscript; available in PMC: 2014 Aug 18.

Published in final edited form as: Curr Protoc Microbiol. 2013;0 14:Unit14D.5. doi: 10.1002/9780471729259.mc14d05s29

Set up for stereotactic surgery. a) The stereotactic frame is assembled for surgery, and the injector and needle are attached attached. b) The animal is positioned in the stereotactic frame. The ear bars are positioned on either side of the head of the animal. The nose bar and ear bars are tightened securely. The head should be level and completely immobilized. The arrow indicates the bregma, the zero point for stereotactic coordinates.

Set up for stereotactic surgery. a) The stereotactic frame is assembled for surgery, and the injector and needle are attached attached. b) The animal is positioned in the stereotactic frame. The ear bars are positioned on either side of the head of the animal. The nose bar and ear bars are tightened securely. The head should be level and completely immobilized. The arrow indicates the bregma, the zero point for stereotactic coordinates.