| DI water (mL) | 750 | 375 | 188 |
| 10M NaOH (mL) | 0.600 | 0.300 | 0.150 |
| Paraformaldehyde powder (g) | 40 | 20 | 10 |
| 10X PBS (mL) | 100 | 50 | 25 |
| 12M HCl (mL) | Adjust to pH 7.2–7.4 | ||
| Final Volume (mL) | 1000 | 500 | 250 |
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