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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Jun 25;74:330–339. doi: 10.1016/j.yjmcc.2014.06.010

Figure 2. Gap junction channels formed by V85I or L229M had normal or mildly altered Vj-dependent gating and single channel conductances.

Figure 2

(A) Plots show the relationships between normalized junctional conductance (Gj) and transjunctional voltage (Vj) for homomericwtCx40 (black), V85I (light gray) and L229M (dark gray) gap junctions. The Boltzmann parameters for the fitted curves are listed in Supplemental Table 1 (B) Whole cell currents (I1 and I2) were recorded simultaneously for 15 sec. from a pair of N2a cells expressing V85I during a 40 mV Vj step applied to cell 1. Current amplitudes are indicated by dashed lines in the I2 trace (Ij = −ΔI2). (C) Junctional current-voltage (Ij–Vj) relationship for V85I channels was generated from channel current amplitudes determined by Gaussian fits of the all points current histogram (not shown) for each 30 s Vj pulse. The mean slope conductance (γj) was 159 ± 2 pS. (D) Whole cell currents are shown for a 30 sec. recording of a L229M channel during a +40 mV Vj pulse. (E) The Ij−Vj relationship for L229M is shown. The mean slope γ j was 148 ± 2 pS.