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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Anat Rec (Hoboken). 2014 Sep;297(9):1596–1603. doi: 10.1002/ar.22964

Figure 2.

Figure 2

In situ hybridization patterns of myotilin expression in sections of different stages of chicken embryonic hearts. In all panels scale bar = 100 μm. Myo, myocardium; Endo, endocardium; AS, antisense RNA probe.

A. In an HH stage 15 embryo the myocardium (Myo) expresses a relatively high level of myotilin as compared with the endocardium (Endo). AS = anti-sense.

B. Section of an embryonic day 7 (HH stage 31) sense control.

C. Section of day 7 embryo hybridized with antisense probes (AS). As compared to the control section in panel B, only a low level of signal is detectable in the myocardium.

D. A slightly higher level of myotilin expression is apparent in the compact layer and trabeculae of the embryonic day 12 (HH 38) heart.