FIG 7.

Cells lacking DUN1 display defects in dATP and dCTP synthesis during iron scarcity. Wild-type BY4741 and dun1Δ (SPY350) cells were incubated in SC medium or SC medium with 100 μM BPS for 7 h, and dATP and dCTP levels were determined as described in Materials and Methods. The average and standard deviation from three independent biological replicates are represented. The values are relative to the levels obtained for the wild-type strain grown in SC medium. The asterisk indicates statistically significant (P < 0.05) differences between dNTP levels of wild-type cells grown in BPS and the rest of the conditions assayed.