TABLE 1.
Yeast strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Invitrogen |
| SPY386 | BY4741 fet3::URA3 fet4::KanMX4 | 52 |
| SPY122 | BY4741 cth1::KanMX4 cth2::HisMX6 | 35 |
| AXY1928 | BY4741 dun1::KanMX4 fet3::URA3 fet4::KanMX4 | This study |
| SPY350 | BY4741 dun1::KanMX4 | Invitrogen |
| Y300 | MATa can1-100 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 | 64 |
| DES460 | Y300 TRP1::GAP-RNR1 | 65 |
| DES459 | Y300 TRP1::GAP-RNR1 mec1::HIS3 | 65 |
| DES453 | Y300 TRP1::GAP-RNR1 rad53::HIS3 | 65 |
| MHY307 | Y300 TRP1::GAP-RNR1 dun1::HIS3 | This study |
| YWO0607 | MATa ura3 leu2-3,112 his3-11,15 Gal+ | D. H. Wolf |
| YWO0608 | YWO0607pre1-1 | D. H. Wolf |
| BY4743 | MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 LYS2/lys2Δ0 ura3Δ0/ura3Δ0 | Invitrogen |
| SPY485 | BY4743 rad6::KanMX4 | Invitrogen |
| SPY487 | BY4743 ubr2::KanMX4 | Invitrogen |
| SPY496 | BY4743 pep4::KanMX4 | Invitrogen |
| SCY037 | MATa GFP-SML1::HIS3 | 11 |
| SCY049 | MATa dun1::URA3 GFP-SML1::HIS3 | 11 |
| SCY0136 | SCY049 DUN1-TAF-S10A::G418 | 11 |
| SCY0137 | SCY049 DUN1-TAF-S139A::G418 | 11 |
| SCY0138 | SCY049 DUN1-TAF-T380A::G418 | 11 |
| SPY349 | BY4741 SML1-GFP::HISMX4 | Invitrogen |
| W303-1A | MATa ura3-1 ade21 trp1-1 his3-11,15 leu2-3,112 | R. Lill |
| Gal-NFS1 | W303-1A pNFS1::GAL1-10–HIS3 | 74 |
| Gal-YAH1 | W303-1A pYAH1::GAL1-10–LEU2 | 75 |
| Gal-NBP35 | W303-1A pNBP35::GAL1-10–HIS3 | 42 |
| Gal-NAR1 | W303-1A pNAR1::GAL1-10–HIS3 | 76 |
| Gal-GRX4 | W303-1A pGRX4::GAL-L-natNT2 grx3::LEU2 | 46 |
| SPY28 | BY4741 aft1::KanMX4 | Invitrogen |
| BY4742 | MATα his3Δ1 leu2 Δ0 lys2Δ0 ura3Δ0 | Invitrogen |
| grx3Δ grx4Δ | BY4742 grx3::LEU2 grx4::KanMX4 | 44 |
| AXY1926 | BY4742 dun1::KanMX4 grx3::LEU2 grx4::KanMX4 | This study |
| AXY1988 | BY4741 pGAL1-NFS1-HIS3 | This study |
| AXY2059 | BY4741 pGAL1-NFS1-HIS3 dun1::KanMX4 | This study |
| SPY589 | BY4741 sml1::KanMX4 | Invitrogen |
| AXY2243 | BY4741 sml1::HIS3 fet3::URA3 fet4::KanMX4 | This study |
| AXY2141 | BY4741 sml1::HIS3 dun1::KanMX4 fet3::URA3 fet4::KanMX4 | This study |
| MHY375 | W303-1A sml1::HIS3 | 13 |
| MHY380 | W303-1A sml1::HIS3 rad53::HIS3 | 13 |
| Plasmids | ||
| pRS415 | CEN LEU1 | 77 |
| pRS416 | CEN URA3 | 77 |
| pSP419 | pRS415-CTH2 | 35 |
| pSP476 | pRS416-CTH1 | 35 |
| pMH80 | CEN LEU2 DUN1 | This study |
| pMH62 | 2μm LEU2 DUN1-D328A | This study |
| pSP673 | pRS414-DUN1-FLAG | 9 |
| pSP674 | pRS414-DUN1-R60A-FLAG | 9 |
| pSP675 | pRS414-DUN1-K100A-R102A-FLAG | 9 |
| p413TEF | CEN HIS3 TEFp CYC1t | 58 |
| pSP684 | p413-DUN1(EcoRI) | This study |
| pSP685 | p413-DUN1-R60A | This study |
| pSP686 | p413-DUN1-K100A-R102A | This study |
| pSP692 | p413-DUN1 | This study |
| pSP693 | p413-DUN1-S10A | This study |
| pSP694 | p413-DUN1-S139A | This study |
| pSP695 | p413-DUN1-T380A | This study |
| pAFT1-1up | pRS313-AFT1-1up | J. Kaplan |