Strains |
|
|
BY4741 |
MATa
his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
|
Invitrogen |
SPY386 |
BY4741 fet3::URA3 fet4::KanMX4
|
52 |
SPY122 |
BY4741 cth1::KanMX4 cth2::HisMX6
|
35 |
AXY1928 |
BY4741 dun1::KanMX4 fet3::URA3 fet4::KanMX4
|
This study |
SPY350 |
BY4741 dun1::KanMX4
|
Invitrogen |
Y300 |
MATa
can1-100 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1
|
64 |
DES460 |
Y300 TRP1::GAP-RNR1 |
65 |
DES459 |
Y300 TRP1::GAP-RNR1 mec1::HIS3 |
65 |
DES453 |
Y300 TRP1::GAP-RNR1 rad53::HIS3 |
65 |
MHY307 |
Y300 TRP1::GAP-RNR1 dun1::HIS3 |
This study |
YWO0607 |
MATa ura3 leu2-3,112 his3-11,15 Gal+
|
D. H. Wolf |
YWO0608 |
YWO0607pre1-1
|
D. H. Wolf |
BY4743 |
MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 LYS2/lys2Δ0 ura3Δ0/ura3Δ0
|
Invitrogen |
SPY485 |
BY4743 rad6::KanMX4
|
Invitrogen |
SPY487 |
BY4743 ubr2::KanMX4
|
Invitrogen |
SPY496 |
BY4743 pep4::KanMX4
|
Invitrogen |
SCY037 |
MATa
GFP-SML1::HIS3
|
11 |
SCY049 |
MATa
dun1::URA3 GFP-SML1::HIS3
|
11 |
SCY0136 |
SCY049 DUN1-TAF-S10A::G418
|
11 |
SCY0137 |
SCY049 DUN1-TAF-S139A::G418
|
11 |
SCY0138 |
SCY049 DUN1-TAF-T380A::G418
|
11 |
SPY349 |
BY4741 SML1-GFP::HISMX4
|
Invitrogen |
W303-1A |
MATa
ura3-1 ade21 trp1-1 his3-11,15 leu2-3,112
|
R. Lill |
Gal-NFS1 |
W303-1A pNFS1::GAL1-10–HIS3
|
74 |
Gal-YAH1 |
W303-1A pYAH1::GAL1-10–LEU2
|
75 |
Gal-NBP35 |
W303-1A pNBP35::GAL1-10–HIS3
|
42 |
Gal-NAR1 |
W303-1A pNAR1::GAL1-10–HIS3
|
76 |
Gal-GRX4 |
W303-1A pGRX4::GAL-L-natNT2 grx3::LEU2
|
46 |
SPY28 |
BY4741 aft1::KanMX4
|
Invitrogen |
BY4742 |
MATα his3Δ1 leu2 Δ0 lys2Δ0 ura3Δ0
|
Invitrogen |
grx3Δ grx4Δ
|
BY4742 grx3::LEU2 grx4::KanMX4
|
44 |
AXY1926 |
BY4742 dun1::KanMX4 grx3::LEU2 grx4::KanMX4
|
This study |
AXY1988 |
BY4741 pGAL1-NFS1-HIS3
|
This study |
AXY2059 |
BY4741 pGAL1-NFS1-HIS3 dun1::KanMX4
|
This study |
SPY589 |
BY4741 sml1::KanMX4
|
Invitrogen |
AXY2243 |
BY4741 sml1::HIS3 fet3::URA3 fet4::KanMX4
|
This study |
AXY2141 |
BY4741 sml1::HIS3 dun1::KanMX4 fet3::URA3 fet4::KanMX4
|
This study |
MHY375 |
W303-1A sml1::HIS3
|
13 |
MHY380 |
W303-1A sml1::HIS3 rad53::HIS3
|
13 |
Plasmids |
|
|
pRS415 |
CEN LEU1
|
77 |
pRS416 |
CEN URA3
|
77 |
pSP419 |
pRS415-CTH2 |
35 |
pSP476 |
pRS416-CTH1 |
35 |
pMH80 |
CEN LEU2 DUN1
|
This study |
pMH62 |
2μm LEU2 DUN1-D328A
|
This study |
pSP673 |
pRS414-DUN1-FLAG |
9 |
pSP674 |
pRS414-DUN1-R60A-FLAG |
9 |
pSP675 |
pRS414-DUN1-K100A-R102A-FLAG |
9 |
p413TEF |
CEN HIS3 TEFp CYC1t
|
58 |
pSP684 |
p413-DUN1(EcoRI) |
This study |
pSP685 |
p413-DUN1-R60A |
This study |
pSP686 |
p413-DUN1-K100A-R102A |
This study |
pSP692 |
p413-DUN1 |
This study |
pSP693 |
p413-DUN1-S10A |
This study |
pSP694 |
p413-DUN1-S139A |
This study |
pSP695 |
p413-DUN1-T380A |
This study |
pAFT1-1up
|
pRS313-AFT1-1up
|
J. Kaplan |