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. 2014 Sep;34(17):3305–3320. doi: 10.1128/MCB.00677-14

FIG 3.

FIG 3

Histological and biochemical evidence of steatohepatitis and fibrosis in the livers of Nrf2−/− mice fed a high-fat diet. (A) Representative H&E-stained images of livers from Nrf2+/+ and Nrf2−/− mice after 24 weeks on an RC or HF diet from 8 to 10 weeks of age. (B and C) Representative images of reticulin staining (B) and Van Gieson's staining (C) in livers from Nrf2−/− mice after 24 weeks on an RC or HF diet. Bars, 100 μm. (D) ALT activity in plasma of Nrf2+/+ and Nrf2−/− mice after 24 weeks on an RC or HF diet (8 to 11 mice per group). (E) Magnetic resonance analysis of fat content in livers from Nrf2+/+ and Nrf2−/− mice on an RC or HF diet (12 or 13 mice per group). (F) Representative electron microscope images of liver sections from Nrf2+/+ and Nrf2−/− mice on an RC or HF diet; bars, 1,731 nm. (G) Cholesterol levels in plasma from Nrf2+/+ and Nrf2−/− mice on RC and HF diets (9 to 11 mice per group). (D, E, and G) White bars, RC fed; black bars, HF fed. The results are means and SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.