FIG 6.

ER stress pathways are perturbed in the livers of Nrf2^−/− mice. (A) Representative immunoblots from analysis of ER stress and UPR-associated proteins in livers of Nrf2^+/+ and Nrf2^−/− mice on an RC or HF diet. Quantification of the immunoblot data for each protein is shown by genotype and diet (3 mice per group). (B) Quantitative-PCR analysis of mRNAs of ER stress markers from livers of Nrf2^+/+ and Nrf2^−/− mice on RC and HF diets (5 to 8 mice per group). In each case, the data were normalized with respect to RC-fed Nrf2^+/+ levels for each protein and gene. White bars, RC fed; black bars, HF fed. The results are means and SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.