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. 2014 Sep;34(17):3182–3193. doi: 10.1128/MCB.01580-13

FIG 3.

FIG 3

lncRNA MRUL depletion leads to increased chemosensitivity of MDR GC sublines to P-gp related drugs. (A) MRUL relative expression levels. Following the transfection of SGC7901/ADR and SGC7901/VCR cells with siMRUL for 3 days, RT-PCR was used to assess MRUL expression levels and GAPDH was used as the internal control. (B and C) Survival rates of SGC7901/ADR and SGC7901/VCR cells treated with siMRUL or siNC in the presence of ADR (B-a), VCR (B-b), CDDP (C-a), or 5-FU (C-b). The MTT assay was used to examine cell survival rates at the designated time. (D) Plating colony formation assay of SGC7901/ADR and SGC7901/VCR cells treated with siMRUL or siNC in the presence of ADR or VCR. (E) Apoptosis rates of SGC7901/ADR and SGC7901/VCR cells treated with siMRUL or siNC in the presence of ADR, CDDP, or VCR. Data represent means plus standard deviations of three replicates (***, P < 0.001).