TABLE 1.
Primers used for real-time RT-PCRa
| Gene | Sequence |
Size (bp) | |
|---|---|---|---|
| Forward primer | Reverse primer | ||
| MRUL | ACCCACAGACAACTGTGGACCC | GCCGCCCCTATTGTTGCCCA | 225 |
| P-gp | GGCTGATTGGCTGGGCAGGAA | TGGAACGGCCACCAAGACGTG | 203 |
| CROT | ACCTTGAATCAGTCACCAGAAC | CCACCACTCTTCCAGCCAAT | 236 |
| GRM3 | CAGTTGAGTCGCGAGTACGG | TCGGTGAATTTCGGAGTGGG | 249 |
| KIAA1234L | GAGTTGTCGGTGCGAGGAAT | CATGGCTTTGGCCTGGTAGA | 314 |
| ABCB4 | TGTGCAGAAGGCACACATCT | TGACCATCGAGAAGCACTGTC | 504 |
| Uc002hfi | GCTGATGGCTCCACATCTT | TTTTTTTTTTCCTTAACCTTG | 206 |
| NR_026673 | CTGGTGTGAACTTCCGGATT | CAAATGACCACTGCTGGATG | 175 |
| NR_015379 | TTTGCCAGCCTCAGCTTAAT | TTGTCCCCATTTTCCATCAT | 186 |
| NR_027241 | GGGATCTGGGTTTGCTGATA | CCGAATCTTGTGCAGATTGA | 170 |
| Uc003wig | AGGGTTTCAGGTGACCACAG | CACTTGACCCTGCACTCTGA | 192 |
| Uc003whs | CAGTCTGGGCAACAGAGTGA | ACTCAGAGATGCCTGCCAGT | 219 |
| Uc001vtj | TAGGGTTTGGGGCTAGTGTG | CCTGCGCATGACTGTAAAAA | 160 |
| Uc002jmb | TCCTACTGACCCCACCTGAG | CTGGAGACCCAAGAAATCCA | 227 |
| NR_026867 | GAGGATGTGGCTGTGGATTT | CCAGTTGAGGGGTGTCAAGT | 241 |
| NR_027339 | CATCATGCTTTTCCAGCTCA | TGTGGCAAATGAGGAATCAA | 156 |
| NR_027282 | GCACACAGGCATCTAGTGGA | GCTTGGTGGTGTTTTCCTGT | 243 |
| Uc002rvu | TGGTGGGACTTCTGGGTAAG | ATCGTCCTCATCCTCCTCCT | 165 |
| Uc003dhh | ATTCACGGTTATGCCTTTGC | CGTTTCCCTTTGTCCACTGT | 230 |
| Uc010jya | AGACAGCAGCAACAGCTCAA | TTTCATGCCACAACACCAGT | 191 |
| Uc001jtu | TCCCTGCTGGAGTCAGAAGT | TGCTGTCATCTCACGGTCTC | 179 |
| Uc001ras | CCAGTTCACCACTGGAAGGT | TCACAAAGCCTGCATCAAAG | 215 |
| PCNA | CAGGGCTCCATCCTCAAGAA | GAGTCCATGCTCTGCAGGTT | 104 |
| GAPDH | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA | 155 |
a
Melting temperature for all primers was 60°C.