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. 2014 Sep;34(17):3229–3243. doi: 10.1128/MCB.00737-14

FIG 11.

FIG 11

Analysis of E2F target genes reveals expression changes in prometastatic genes with E2F loss. (A) Informatics pipeline for filtering candidate genes for qRT-PCR testing. (B to J) Testing potential target genes via qRT-PCR, we found that E2F1−/− and E2F2−/− tumors (six of each type were analyzed) have significantly lower levels of Bmp4 (P, 0.0002 and <0.0001, respectively) (B), Cyr61 (P, 0.0009 and 0.0006, respectively) (C), Nupr1 (P, <0.0001 for both types) (D), Plod 2 isoform 1 (P, <0.0001 for E2F2−/− tumors only) (E), Plod 2 isoform 2 (P, 0.0122 and 0.0015, respectively) (F), P4ha1 (P, 0.0006 and <0.0001, respectively) (G), Adamts1 (P, <0.0001 for both types) (H), Lgals3 (P, <0.0001 for both types) (I), and Angpt2 (P, 0.0065 for E2F1−/− tumors only) (J).