FIG 3.

Fibroblasts of S. granarius contain active telomerase. (A and B) Conventional TRAP analysis of S. granarius fibroblast cells revealed telomerase activity in primary and long-term-culture cells. (A) DNA PAGE gel after conventional TRAP using protein extracts from S. granarius fibroblasts at different passages. A protein extract from PHA-stimulated human leukocytes was used as a positive control. (B) Densitometric analysis of conventional TRAP results. (C) A real-time semiquantitative TRAP assay was performed on cell extracts prepared from early-passage S. granarius cells (passage 9) and compared to the activity found in an equal number of human HeLa cells. Serial dilutions of S. granarius whole-cell extracts (3,000, 1,000, and 300 cell equivalents) resulted in a decreased TRAP activity. Treatment with RNase A and omission of the cell extract served as negative controls.