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Telomeric circles are detected in primary S. granarius fibroblasts. Ten micrograms of genomic DNA from S. granarius early-passage (p13) fibroblasts (A) and 20 μg genomic DNA from the human ALT cancer cell line U2OS (B) were digested with MboI, separated by 2D gel electrophoresis, transferred onto N+ nylon membranes, and hybridized with a digoxigenin-labeled telomeric C-rich oligonucleotide. Arrow 1, single-stranded linear DNA; arrow 2, double-stranded linear DNA; arrow 3, circular DNA.
