TABLE 2.

Frequencies of T-SCEs in primary S. granarius fibroblast cells (passages 4 to 8)^a

Culture type and expt no.	No. of metaphase spreads analyzed	No. of extremities with long telomeres	No. of metaphase spreads with:		% of metaphase spreads with T-SCEs (±SEM)
			1 T-SCE	>1 T-SCE
Asynchronous
1	86	2,738	40	5	52.3
2	90	2,870	43	7	55.6
3	70	2,216	37	4	58.6
Total	246	7,824	120	16	55.5 (±3.8)
Synchronous
1	100	3,191	42	7	49.0
2	90	2,872	43	8	56.6
Total	190	6,063	85	15	52.3 (±4.8)

^aT-SCEs in primary S. granarius fibroblasts were detected using one-color CO-FISH. Chromosome material was prepared from both asynchronous (three independent experiments) and synchronized (serum starvation; two independent experiments) cell cultures.