FIG 1.
Proteomic analysis of differentially tyrosyl-phosphorylated proteins in hearts of Ptpn11D61G/+ mice. (A) Classification of hypo- and hyper-tyrosyl-phosphorylated proteins in the hearts of Ptpn11D61G/+ mice. (B) Log2-transformed values for the ratio of each phosphotyrosine-containing peptide in wild-type and Ptpn11D61G/+ mouse hearts. (C) Heat map of differentially tyrosyl-phosphorylated peptides (the site of phosphorylation is identified by MS in parentheses). (D) Extracted ion chromatogram and peptide sequence of PZR-containing tyrosine 242 (upper panels) and tyrosine 264 (lower panels) by differential proteomics. (E) Amino acid sequences of the PZR C terminus in different vertebrates. Consensus sequences for ITIM (S/I/V/LXYXXI/V/L) are indicated in boldface, and tyrosine residues are marked red with the appropriate amino acid numbering. Sequences are shown for the PZR C terminus from Homo sapiens, Mus musculus, Rattus norvegicus, Bos taurus, Canis lupus familiaris, Danio rerio, Gallus gallus.