FIG 1.

Conditioned medium of human MSCs reduces the proportion of lung cancer stem cells. Lung cancer cells (H1299, A549, and H1975), breast cancer cells (MCF-7), and cervical cancer cells (HeLa) were cultured for 48 h with conditioned medium of MSCs (MSC CM) or mock-conditioned medium (control CM). After the culture, cells were stained with Hoechst 33342 or 7-AAD–pyronin Y to analyze the side population (A and B) or cell cycle (C and D), respectively. (A) Representative side population subset (SP) with the percentage in H1299 cells. (B) Proportion of cancer cells in the side population. (C) Representative phase distribution of the cell cycle with the percentage of H1299 cells in the G₀ phase. (D) Proportion of cancer cells in the G₀ phase. For panels B and D, the cell proportion is reported as the mean percentage ± standard error per group (n = 3). Asterisks indicate significant differences compared with the control CM.