FIG 6.

MSCs produce FGF10 affecting lung cancer stem cells. (A) Western blot analysis of FGF10 in transwell-cocultured MSCs with H1299 or A549 lung cancer cells. Controls included MSCs cultured without lung cancer cells. MSCs were from three unrelated donors, and β-actin production (ACTB) was used as a control. (B and C) Functional analysis of FGF10 production. The side population was analyzed in cancer cells stained with Hoechst 33342 after the 48-h culture with conditioned medium of MSCs in the presence of anti-FGF10 neutralizing antibody or control antibody. A representative side population subset (SP) with the percentage in H1299 lung cancer cells (B) and proportions of five cancer cells in the side population (C) are shown. The cell proportion is reported as the mean percentage ± standard error per group (n = 3). Asterisks indicate significant differences compared with the control antibody.