FIG 8.

FGF10 suppresses reproliferation after an anticancer treatment. A549 lung cancer cells were treated with the anticancer drug cisplatin (CDDP) in the presence of recombinant FGF10 or vehicle (PBS [control]) for 2 days. (A) Time course of reproliferation after the anticancer treatment. The cell density relative to that at start of the experiment (day 0, indicated by the dotted horizontal line) was monitored by a colorimetric assay using MTS. (B) OCT3/4, SOX2, and LGR4 gene expression in A549 cells on day 4. The level of expression relative to that on day 0 was analyzed by quantitative RT-PCR. Data are presented as the mean ± standard error per group (n = 3). Asterisks indicate significant differences compared with the control vehicle.