FIG 6.
Zfp521 suppresses adipocyte commitment. (A) Oil red O staining of W20 cells expressing scrambled or Zfp521 shRNA differentiated in adipogenic medium supplemented or not with BMP2 for 6 days. (B and C) qRT-PCR of adipocyte selective genes (B) or osteogenic selective genes (C) in the differentiated cultures shown in panel A. (D) In vivo gene expression of key adipocyte commitment factors within the BMP2-induced newly formed bone of wild-type and Zfp521cko mice. Mice were injected with BMP2 as indicated, and qRT-PCR was performed on mRNA isolated directly from newly formed bone tissue 24 h after the final injection. The data are presented as means ± SD; n ≥ 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not significant relative to shScr within the same vehicle or BMP2 treatment group; Student's t test.