FIG 5.
Induction of CAV1 does not increase EGFR signaling or cell growth. (A) Western analysis of phospho-EGFR (pEGFR), total EGFR, phospho-ERK (pERK), and total ERK signaling in the colons of Hif2αLSL/LSL and littermate control (wild-type) mice. (B) 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay in HCT116 cells with CAV1 overexpression in medium containing 0.5% or 10% FBS. N.S., not significant. (C) Western blot of CAV1 expression in HCT116 cells infected with adenovirus expressing CAV1. Ad-CAV1, adenovirus expressing CAV1; Ad-GFP, adenovirus expressing green fluorescent protein.