FIG 6.

HIF2α in the colon decreased occludin expression and increased colon barrier permeability. (A) Occludin expression level in the colons of Vhl^ΔIE and Vhl^ΔIE/Hif2α^ΔIE mice. (B) Occludin staining in colon sections from Vhl^ΔIE and Vhl^flox/flox mice. (C) FITC-dextran colon barrier permeability assay in Vhl^ΔIE and Vhl^flox/flox mice which were untreated, treated with DSS for 3 days, or treated with DSS for 3 days and placed back on regular drinking water for 6 days. (D) FITC-dextran colon barrier permeability assay in untreated Vhl^ΔIE/Hif2α^ΔIE and Vhl^flox/flox/Hif2α^flox/flox mice. (E) CAV1 and occludin expression level in the colons of Hif2α^LSL/+ mice or their littermate controls (wild type). (F) FITC-dextran colon barrier permeability assay in Hif2α^LSL/+ mice pretreated with infliximab. Four to six mice were assessed per each group. Each bar represents the mean value ± SD. The results for bars with different letters are statistically significantly different (P < 0.05); N.S., not significant.